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Antibodies certain to the blocking peptide no more bind on the epitope within the goal protein. By comparing the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.

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This guide outlines further attainable optimization of cellular and immunoassay parameters to be certain the very best final results are...

This guide outlines more feasible optimization of cellular and immunoassay parameters to be certain the very best success are...

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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...

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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide commonly contains the epitope recognized through the antibody.

Focus on Information and facts Cyclin E belongs to the hugely conserved cyclin family members, whose associates are characterized by a dramatic periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which add to your temporal coordination of each and every mitotic party. Cyclin E varieties a fancy with and functions as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and it is degraded as cells development via S section.

Overexpression of this gene has long been observed in many tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant part in endorsing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two supplemental splice variants ended up reported but comprehensive nucleotide sequence details is just not but offered.

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